Borussia Dortmund striker Paco Alcacer (pictured) and Wolves defender Jonny Castro are among those included in Luis Enrique’s Spain squad.
La Roja face Wales in a friendly before hosting England in the UEFA Nations League in Seville in a duo of October fixtures.
Alcacer has netted six times in 13 international caps but the last of those goals came in 2015 and has already scored three times for the Bundesliga club despite making just one start.
The 25-year-old joined Dortmund on a season-long loan from Barcelona, after the Catalan giants sanctioned his loan exit to allow him more first-team opportunities.
Defender Jonny is on a similar season-long loan deal in England after joining Wolves from Atletico Madrid, who signed him on a permanent deal from Celta Vigo this summer.
The 24-year-old full-back has played in all seven league games for the Midlands outfit this season and is in the Spain squad for the first time having previously starred at Under-21 level.
Real Betis defender Marc Bartra is also included in the squad after starring since his January move from Dortmund, while Atletico Madrid midfielder Koke is back in the squad.
Barcelona full-back Jordi Alba is still excluded from the squad while Diego Costa is among those to drop out after patchy form for Atletico while Real Madrid duo Dani Carvajal and Isco both miss out through injury.
Spain 23-man squad
Goalkeepers: Kepa, Pau Lopez, De Gea
Defenders: Gayá, Marcos Alonso, Albiol, Nacho, Ramos, Bartra, Azpilicueta, Jonny
Midfielders: Busquets, Rodri, Koke, Ceballos, Thiago, Saúl
Forwards: Asensio, Morata, Rodrigo, Suso, Iago Aspas, Paco Alcacer