Alex Song and Aly Cissokho are both handed first starts for their new clubs tonight as Barcelona host Valencia.
Puyol, with a fractured cheekbone, is named on the bench, with Javier Mascherano starting alongside Gerard Pique in defence.
Full-back Dani Alves is fit enough to start after his thigh injury in midweek, whilst midfielder Song has Xavi Hernandez and Cesc Fabregas for company in his first start for the club. Pedro Rodriguez is selected ahead of Cristian Tello.
Meanwhile, visitors Valencia have Adil Rami in place of the suspended Ricardo Costa and hand a debut to Aly Cissokho at left-back. David Albelda comes in for Fernando Gago, who only makes the bench after illness.
Barcelona: Valdes; Alves, Pique, Mascherano, Adriano; Cesc, Xavi, Song; Alexis, Messi, Pedro
Valencia: Diego Alves; Pereira, Rami, V. Ruiz, Cissokho; Feghouli, T.Costa, Albelda, Guardado; Jonas, Soldado