Luis Enrique has rested a number of first team players for the Copa del Rey trip to Villanovense, including Neymar and Luis Suarez.
Barca go to the Extremadura-based Segunda B side on Wednesday, playing their first domestic Cup tie of the season earlier than the rest of La Liga due to their involvement in the Club World Cup in December.
The Catalans have confirmed a much-changed squad for the game at Romero Cuerda, with Andres Iniesta, Neymar, Sergio Busquets, Javier Mascherano, Gerard Pique, Claudio Bravo and Suarez all staying home.
In their place are youngsters Sergi Samper and Gerard Gumbau, who have been on the fringes of the Barca first team, and Wilfrid Kaptoum and Aitor Cantalapiedra, a pair of midfielders.
Barcelona squad to play Villanovense:
Ter Stegen, Masip, Alves, Douglas, Bartra, Vermaelen, Mathieu, Alba, Adriano, Rakitic, Samper, Gumbau, Kaptoum, Aitor, Sandro, Munir