Juan Ignacio Martinez names Javi Guerra in attack against a Villarreal side with Ikechukwu Uche up front.
Villarreal Coach Marcelino partners Uche with Giovani dos Santos as the Yellow Submarine seek three points to go fourth.
A win for the visitors would see Villarreal go above Athletic at least for 24 hours, with Athletic not playing Osasuna until Sunday.
Valladolid need the victory to draw level with Almeria at the other end of the table.
Valladolid: Marino; Rukavina, Rueda, Mitrovic, Pena; Alvaro Rubio, Rossi; Larsson, Oscar, Rama; Javi Guerra
Villarreal: Asenjo; Mario, Musacchio, Dorado, Jaume Costa; Pina, Bruno Soriano; Aquino, Moi Gomez; Uche Giovani dos Santos