Real Madrid will be wary of suffering a reprise of last season's biggest shock result against Levante.
The Merengues fell to a 1-0 defeat at the Estadio Ciutat de Valencia in September last year, Arouna Kone the Granotes hero.
Kone and many others have since left but Valencia's second club are somehow still thriving, sitting sixth in La Liga and well on course for qualification from the Europa League group stage.
“We have taken an important step but it's a contained joy because we haven't achieved anything yet,” said Juan Ignacio Martinez after Thursday's 0-0 draw with Twente.
“Now we must think of our next game against Real Madrid and prepare for that, but playing against Madrid is a great motivation in itself.”
Jose Mourinho will have to play Cristiano Ronaldo up front, as strikers Karim Benzema and Gonzalo Higuain are both injured. Real are third, just five points behind Atletico Madrid now after their neighbours slip up last weekend.
Kick off: Sunday November 11th, 8.30 (BST)