Neymar and his father are facing a corruption trial after a Spanish judge re-opened the case into his 2013 transfer to Barcelona from Santos.
Barca president Josep Maria Bartomeu and his predecessor Sandro Rosell will also be implemented in the trial after DIS - the Brazilian investment group which owned 40% of the attacker's sporting rights - alleged they received less money than what they were entitled to, due to part of the deal being concealed.
Judge Jose De La Mata requested a trial at Spain's National Court, and prosecutors now have ten days to formalise that request.
"The Board of Directors have decided to appeal against the interlocutory judgement made by judge José de la Mata in Madrid," a Barcelona club statement read.
"He has proposed that the National Court open an indictment against the player, Neymar Jr, his parents, ex-president Sandro Rosell, president Jose Maria Bartomeu, and the club as a juridical person. This is in the context of the lawsuit interposed by the investment fund DIS because of the signing of the Brazilian player.
"The board expressed its surprise and total disagreement with this development and its reasoning, especially taking into account the same magistrate's decision to dismiss the case last July and no action took place against the president Bartomeu. If the judicial action starts, FC Barcelona will maintain the innocence of those who are being investigated."
Neymar had appeared in court in February and De La Mata ruled that FIFA regulations 'were not properly followed' but that it was an issue for a civil court, not a criminal court.
See the latest La Liga predictions and betting tips with Eurotips.co.uk