The two players were included in the matchday squad to face the Italian side in Saturday’s Round of 16 second leg encounter at the Camp Nou, which the Blaugrana won 3-1.
It was expected that both would play a part with many first-team players unavailable, such as Sergio Busquets, Arturo Vidal, Arthur Melo, Ousmane Dembele and Martin Braithwaite.
The club are said to be so bemused as Setien made a stand of wanting the duo included for the squad and for their preparation not to be disrupted by playing for the side’s B team, who lost their promotion playoff match to Sabadell.
Barcelona have subsequently lost approximately €10m due to their B side failing to win promotion to the Segunda through TV revenue.
What was even more surprising was that B team captain Monchu – who played in the playoff fixtures – was one of the substitutes introduced during Saturday night’s game.
Setien said after the game when asked why the Ansu and Puig did not feature: “Given the circumstances, we thought it was appropriate (not to field them).”