The Spanish Football Federation [RFEF] have rejected Gary Medel's appeal against his two-match ban.
The Sevilla man was sent off last weekend in the derby match against Real Betis, but the governing body have decided to uphold their initial sanction.
Medel produced a dangerous challenge on Jose Canas which the Federation deemed to be 'violent'.
The Chile international will miss matches against Atletico Madrid and Real Valladolid.
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